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Feruloyl esterase (rumen microorganism)

Feruloyl esterase rumen microorganism E-FAERU
Product code: E-FAERU
€152.00

1,000 Units

Prices exclude VAT

Available for shipping

Content: 1,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Esterase
EC Number: 3.1.1.73
CAZy Family: CE1
CAS Number: 134712-49-5,
224306-54-1,
224306-55-2
Synonyms: feruloyl esterase; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase
Source: Rumen microorganism
Molecular Weight: 29,000
Concentration: Supplied at ~ 400 U/mL
Expression: Recombinant from Rumen microorganism
Specificity: Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates.
Specific Activity: ~ 30 U/mg (40oC, pH 6.5 on ethyl ferulate)
Unit Definition: One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one µmole of ferulic acid per minute from ethyl-ferulate (0.39 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC.
Temperature Optima: 40oC
pH Optima: 7
Application examples: Applications established in biofuels, paper and pulp, food, nutrition, medical and pharmacological industries.

High purity recombinant Feruloyl Esterase (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Find more Carbohydrate Active enZYmes products for various applications.

 

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
The role of activity coefficients in bioreaction equilibria: Thermodynamics of methyl ferulate hydrolysis.

Hoffmann, P., Voges, M., Held, C. & Sadowski, G. (2013). Biophysical Chemistry, 173, 21-30.

The Gibbs energy of reaction (ΔRg) is the key quantity in the thermodynamic characterization of biological reactions. Its calculation requires precise standard Gibbs energy of reaction (ΔRg+) values. The value of ΔRg+ is usually determined by measuring the apparent (concentration-dependent) equilibrium constants K, e.g., the molality-based Km. However, the thermodynamically consistent determination of ΔRg+ requires the thermodynamic (activity-based) equilibrium constant Ka. These values (Km and Ka) are equal only if the ratio of the activity coefficients of the reactants to the activity coefficients of the products (Kγ) is equal to unity. In this work, the impact of Kγ on the estimation of Ka for biological reactions was investigated using methyl ferulate (MF) hydrolysis as a model reaction. The value of Kγ was experimentally determined from Km values that were measured at different reactant concentrations. Moreover, Kγ was independently predicted using the thermodynamic model ePC-SAFT. Both the experimentally determined and the predicted Kγ values indicate that this value cannot be assumed to be unity in the considered reaction. In fact, in the reaction conditions considered in this work, Kγ was shown to be in the range of 3 < Kγ < 6 for different reactant molalities (2 < mmol MF kg-1 < 10). The inclusion of Kγ and thus the use of the thermodynamically correct Ka value instead of Km lead to remarkable differences (almost 40%) in the determination of ΔRg+. Moreover, the new value for ΔRg+ increases the concentration window at which the reaction can thermodynamically occur. The influence of additives was also investigated both experimentally and theoretically. Both procedures consistently indicated that the addition of NaCl (0 to 1 mol kg-1 water) moderately decreased the value of Kγ, which means that the values of Km increase and that a higher amount of products is obtained as a result of the addition of salt. Additionally, Km was found to strongly depend on pH. A ten-fold increase in the Km values was observed in the pH range of 6 to 7; this increase corresponds to a change of more than 100% in the value of ΔRg+.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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