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Glucuronoyl Esterase (Ruminococcus flavefaciens)

Product code: E-GERF
€156.00

250 Units

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Content: 250 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: Esterase
EC Number: 3.1.1.B11
CAZy Family: CE15
CAS Number: 9016-18-6
Synonyms: glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase
Source: Ruminococcus flavefaciens
Molecular Weight: 47,000
Concentration: Supplied at ~ 370 U/mL
Expression: Recombinant from Ruminococcus flavefaciens
Specificity: Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.
Specific Activity: ~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3)
Unit Definition: One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3.
Temperature Optima: 40oC
pH Optima: 7
Application examples: Applications in carbohydrate research and in the food industry.

High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Documents
Certificate of Analysis
Safety Data Sheet
Data Sheet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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