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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||> 4 years at 4oC|
|Synonyms:||arabinan endo-1,5-alpha-L-arabinanase; 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase|
|Concentration:||Supplied at ~ 1,500 U/mL|
|Expression:||Recombinant from Cellvibrio japonicus|
|Specificity:||endo- / exo-hydrolysis of (1,5)-α-arabinofuranosidic linkages in (1,5)-arabinans. Processive mode of action with predominant release of arabinotriose from linear arabinan.|
|Specific Activity:||~ 50 U/mg (40oC, pH 7.0 on debranched arabinan)|
|Unit Definition:||One Unit of arabinananase activity is defined as the amount of enzyme required to release one µmole of arabinose reducing-sugar equivalents per minute from debranched arabinan (5 mg/mL) in potassium phosphate buffer (100 mM), pH 7.0 at 40oC.|
|Application examples:||Applications in carbohydrate and biofuels industry.|
Sansone, M., Sansone, A. C. M. B., Shiga, T. M. & do Nascimento, J. R. O. (2016). Food Research International, 87, 125-133.
Some diet components, such as certain indigestible polysaccharides from edible plants, may interact with the gut-associated lymphoid tissue and improve the host immune response to pathogens. The non-starch polysaccharides (NSP) from bananas are non-digestible carbohydrates that resemble some immunomodulatory polysaccharides occurring in the cell wall of cereals. Based on this similarity, the effects of the water-soluble NSP from two banana cultivars (Nanicão and Thap Maeo) on the phagocytic activity, nitric oxide (NO) and cytokines produced by cultured macrophages were investigated. An investigation into the monosaccharide composition and the oligomers released by enzymatic hydrolysis of the ultra-filtered fraction above 50 kDa and the heat-treated fraction of water-soluble NSP from both cultivars revealed they are mostly composed of mannan and galacturonans (homogalacturonan, xylogalacturonan and rhamnogalacturonan). The NSP tested were able to activate the macrophages, but the effects on the phagocytic activity and the release of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide production were dependent on the polysaccharide concentration and the cultivar origin. Despite some specific differences, the NSP from Nanicão and Thap Maeo banana fruits may be considered prospective food immunomodulators, which contribute to the promotion of a more responsive immune system.Hide Abstract