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endo- / exo-Arabinanase (Cellvibrio japonicus)

Product code: E-ARBACJ
€165.00

3,000 Units

Prices exclude VAT

Available for shipping

Content: 3,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: endo-Arabinanase
EC Number: 3.2.1.99
CAZy Family: GH43
CAS Number: 75432-96-1
Synonyms: arabinan endo-1,5-alpha-L-arabinanase; 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase
Source: Cellvibrio japonicus
Molecular Weight: 37,000
Concentration: Supplied at ~ 1,500 U/mL
Expression: Recombinant from Cellvibrio japonicus
Specificity: endo- / exo-hydrolysis of (1,5)-α-arabinofuranosidic linkages in (1,5)-arabinans. Processive mode of action with predominant release of arabinotriose from linear arabinan.
Specific Activity: ~ 50 U/mg (40oC, pH 7.0 on debranched arabinan)
Unit Definition: One Unit of arabinananase activity is defined as the amount of enzyme required to release one µmole of arabinose reducing-sugar equivalents per minute from debranched arabinan (5 mg/mL) in potassium phosphate buffer (100 mM), pH 7.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 7
Application examples: Applications in carbohydrate and biofuels industry.

High purity recombinant endo- / exo-Arabinanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

See Carbohydrate Active enZYmes for more enzyme products.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
The water-soluble non-starch polysaccharides from bananas display immunomodulatory properties on cultured macrophages.

Sansone, M., Sansone, A. C. M. B., Shiga, T. M. & do Nascimento, J. R. O. (2016). Food Research International, 87, 125-133.

Some diet components, such as certain indigestible polysaccharides from edible plants, may interact with the gut-associated lymphoid tissue and improve the host immune response to pathogens. The non-starch polysaccharides (NSP) from bananas are non-digestible carbohydrates that resemble some immunomodulatory polysaccharides occurring in the cell wall of cereals. Based on this similarity, the effects of the water-soluble NSP from two banana cultivars (Nanicão and Thap Maeo) on the phagocytic activity, nitric oxide (NO) and cytokines produced by cultured macrophages were investigated. An investigation into the monosaccharide composition and the oligomers released by enzymatic hydrolysis of the ultra-filtered fraction above 50 kDa and the heat-treated fraction of water-soluble NSP from both cultivars revealed they are mostly composed of mannan and galacturonans (homogalacturonan, xylogalacturonan and rhamnogalacturonan). The NSP tested were able to activate the macrophages, but the effects on the phagocytic activity and the release of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide production were dependent on the polysaccharide concentration and the cultivar origin. Despite some specific differences, the NSP from Nanicão and Thap Maeo banana fruits may be considered prospective food immunomodulators, which contribute to the promotion of a more responsive immune system.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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