The product has been successfully added to your shopping list.

Cellulase (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans)

Product code: E-CELTH

300 Units at 60oC

Prices exclude VAT

This product has been discontinued

Content: 300 Units at 60oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: endo-Cellulase
EC Number:
CAZy Family: GH6
CAS Number: 9012-54-8
Synonyms: 1,4-beta-D-glucan 4-glucanohydrolase
Source: Thermobifida halotolerans
Molecular Weight: 44,000
Concentration: Supplied at ~ 150 U/mL
Expression: Recombinant from Thermobifida halotolerans
Specificity: endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.
Specific Activity: ~ 27 U/mg protein (60oC, pH 8.5 on CM-Cellulose 4M);
~ 16 U/mg protein (40oC, pH 8.5 on CM-Cellulose 4M)
Unit Definition: One Unit of cellulase (endo-1,4-β-D-glucanase) activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in Tris buffer (50 mM), pH 8.5.
Temperature Optima: 60oC
pH Optima: 8.5
Application examples: For use in research.

This product has been discontinued (read more).

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Show more CAZy enzyme products.

Certificate of Analysis
Safety Data Sheet
Data Sheet

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

Rovera, C., Fiori, F., Trabattoni, S., Romano, D. & Farris, S. (2020). Nanomaterials, 10(4), 735.

Bacterial cellulose nanocrystals (BCNCs) obtained by enzymatic hydrolysis have been loaded in pullulan biopolymer for use as nanoparticles in the generation of high-oxygen barrier coatings intended for food packaging applications. Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was hydrolyzed by two different enzymatic treatments, i.e., using endo-1,4-β-glucanases (EGs) from Thermobifida halotolerans and cellulase from Trichoderma reesei. The hydrolytic activity was compared by means of turbidity experiments over a period of 145 h, whereas BCNCs in their final state were compared, in terms of size and morphology, by atomic force microscopy (AFM) and dynamic light scattering (DLS). Though both treatments led to particles of similar size, a greater amount of nano-sized particles (≈250 nm) were observed in the system that also included cellulase enzymes. Unexpectedly, transmission electron microscopy (TEM) revealed that cellulose nanoparticles were round-shaped and made of 4-5 short (150-180 nm) piled whiskers. Pullulan/BCNCs nanocomposite coatings allowed an increase in the overall oxygen barrier performance, of more than two and one orders of magnitude (≈0.7 mL·m−2·24 h−1), of pure polyethylene terephthalate (PET) (≈120 mL·m−2·24 h−1) as well as pullulan/coated PET (≈6 mL·m−2·24 h−1), with no significant difference between treatments (hydrolysis mediated by EGs or with the addition of cellulase). BCNCs obtained by enzymatic hydrolysis have the potential to generate high oxygen barrier coatings for the food packaging industry.

Hide Abstract
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Customers also viewed
Inulin P-INUL
endo-Inulinase Aspergillus niger E-ENDOIAN
endo-Inulinase (Aspergillus niger)
Cellulase endo-1-4-beta-D-glucanase Trichoderma longibrachiatum E-CELTR
Cellulase (endo-1,4-β-D-glucanase) (Trichoderma longibrachiatum)
Cellulase endo-1-4-beta-D-glucanase Talaromyces emersonii E-CELTE
Cellulase (endo-1,4-β-D-glucanase) (Talaromyces emersonii)
Cellulase endo-1-4-beta-D-glucanase Bacillus amyloliquefaciens E-CELBA
Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens)
D-Mannose D-Fructose D-Glucose Assay kit K-MANGL MANGL
D-Mannose/D-Fructose/D-Glucose Assay kit
D-Glucuronic D-Galacturonic Acid Assay Kit K-URONIC URONIC
D-Glucuronic/D-Galacturonic Acid Assay Kit
Available Carbohydrates/Dietary Fiber Assay Kit K-ACHDF ACHDF
Available Carbohydrates/Dietary Fiber Assay Kit