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Cellulase (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans)

Product code: E-CELTH
€152.00

300 Units at 60oC

Prices exclude VAT

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Content: 300 Units at 60oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: endo-Cellulase
EC Number: 3.2.1.4
CAZy Family: GH6
CAS Number: 9012-54-8
Synonyms: 1,4-beta-D-glucan 4-glucanohydrolase
Source: Thermobifida halotolerans
Molecular Weight: 44,000
Concentration: Supplied at ~ 150 U/mL
Expression: Recombinant from Thermobifida halotolerans
Specificity: endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.
Specific Activity: ~ 27 U/mg protein (60oC, pH 8.5 on CM-Cellulose 4M);
~ 16 U/mg protein (40oC, pH 8.5 on CM-Cellulose 4M)
Unit Definition: One Unit of cellulase (endo-1,4-β-D-glucanase) activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in Tris buffer (50 mM), pH 8.5.
Temperature Optima: 60oC
pH Optima: 8.5
Application examples: For use in research.

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication

Enzymatic Hydrolysis of Bacterial Cellulose for the Production of Nanocrystals for the Food Packaging Industry.

Rovera, C., Fiori, F., Trabattoni, S., Romano, D. & Farris, S. (2020). Nanomaterials, 10(4), 735.

Bacterial cellulose nanocrystals (BCNCs) obtained by enzymatic hydrolysis have been loaded in pullulan biopolymer for use as nanoparticles in the generation of high-oxygen barrier coatings intended for food packaging applications. Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was hydrolyzed by two different enzymatic treatments, i.e., using endo-1,4-β-glucanases (EGs) from Thermobifida halotolerans and cellulase from Trichoderma reesei. The hydrolytic activity was compared by means of turbidity experiments over a period of 145 h, whereas BCNCs in their final state were compared, in terms of size and morphology, by atomic force microscopy (AFM) and dynamic light scattering (DLS). Though both treatments led to particles of similar size, a greater amount of nano-sized particles (≈250 nm) were observed in the system that also included cellulase enzymes. Unexpectedly, transmission electron microscopy (TEM) revealed that cellulose nanoparticles were round-shaped and made of 4-5 short (150-180 nm) piled whiskers. Pullulan/BCNCs nanocomposite coatings allowed an increase in the overall oxygen barrier performance, of more than two and one orders of magnitude (≈0.7 mL·m−2·24 h−1), of pure polyethylene terephthalate (PET) (≈120 mL·m−2·24 h−1) as well as pullulan/coated PET (≈6 mL·m−2·24 h−1), with no significant difference between treatments (hydrolysis mediated by EGs or with the addition of cellulase). BCNCs obtained by enzymatic hydrolysis have the potential to generate high oxygen barrier coatings for the food packaging industry.

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Safety Information
Symbol : Not Applicable
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Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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