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4-Nitrophenyl-β-D-glucopyranoside

4-Nitrophenyl-beta-D-glucopyranoside O-PNPBG
Product code: O-PNPBG
€96.00

2 g

Prices exclude VAT

Available for shipping

Content: 2 g
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 2492-87-7
Synonyms: p-Nitrophenyl-β-D-glucopyranoside, pNP-β-D-glucopyranoside
Molecular Formula: C12H15NO8
Molecular Weight: 301.3
Purity: > 98%
Substrate For (Enzyme): β-Glucosidase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-β-D-glucopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of β-glucosidase activity.

Display all available colourimetric oligosaccharides.

Documents
Certificate of Analysis
Safety Data Sheet
FAQs Booklet
Publications
Publication

Innovative microscale workflow from fungi cultures to Cell Wall‐Degrading Enzyme screening.

Raulo, R., Heuson, E., Siah, A., Phalip, V. & Froidevaux, R. (2019). Microbial Biotechnology, 12(6), 1286-1292.

This study aimed at developing a complete miniaturized high‐throughput screening workflow for the evaluation of the Cell Wall‐Degrading Enzyme (CWDE) activities produced by any fungal strain directly cultivated on raw feedstock in a submerged manner. In this study, wheat straw was selected as model substrate as it represents an important carbon source but yet poorly valorised to yield high added value products. Fungi were grown in a microbioreactor in a high‐throughput (HT) way to replace the fastidious shaking flask cultivations. Both approaches were compared in order to validate our new methodology. The range of CWDE activities produced from the cultures was assayed using AZO‐died and pNP‐linked substrates in an SBS plate format using a Biomek FXp pipetting platform. As highlighted in this study, it was shown that the CWDE activities gathered from the microbioreactor cultivations were similar or higher to those obtained from shake flasks cultures, with a lower standard deviation on the measured values, making this new method much faster than the traditional one and suitable for HT CWDE production thanks to its pipetting platform compatibility. Also, the results showed that the enzymatic activities measured were the same when doing the assay manually or using the automated method.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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