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Xylose dehydrogenase + Xylose mutarotase

Xylose dehydrogenase Xylose mutarotase E-XYLMUT
Product code: E-XYLMUT
€157.00

2.5 mL; xylose dehydrogenase (60 U/mL) /
xylose mutarotase (2 mg/mL)

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Content: 2.5 mL; xylose dehydrogenase (60 U/mL) / 
xylose mutarotase (2 mg/mL)
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: In 50% (v/v) glycerol
Physical Form: Solution
Stability: > 2 years below -10oC
Enzyme Activity: Dehydrogenase
EC Number: XDH: 1.1.1.175
XMR: 5.1.3.3
CAS Number: XDH: 62931-20-8
XMR: 9031-76-9  
Synonyms: XDH: D-xylose 1-dehydrogenase; D-xylose:NAD+ 1-oxidoreductase
XMR: Aldose 1-epimerase
Molecular Weight: 26,000
Concentration: Supplied at ~ 60 U/mL
Expression: Recombinant
Specificity: Interconversion of the α- and β-anomeric forms of D-xylose is catalysed by xylose mutarotase (XMR) (1). 

(1) α-D-Xylose ↔ β-D-xylose.

The β-D-xylose is oxidised by NAD+ to D-xylonic acid in the presence of β-xylose dehydrogenase (β-XDH) at pH 7.5 (2).

(2) β-D-Xylose + NAD+ ↔ D-xylonic acid + NADH + H+
Specific Activity: XDH: ~ 60 U/mL at pH 7.5 and 25oC /
XMR: 2 mg/mL
Unit Definition: One Unit of xylose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at 25oC.
Temperature Optima: 25oC
pH Optima: 7.5
Application examples: For the measurement of D-xylose, especially in broths and hydrolysates of plant material and polysaccharides. Refer to the D-XYLOSE Assay Kit booklet (Megazyme cat. no. K-XYLOSE) for directions of use.

High purity recombinant Xylose dehydrogenase + Xylose mutarotase for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication
Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

A range of α-L-arabinofuranosyl-(1-4)-β-D-xylo-oligosaccharides (AXOS) were produced by hydrolysis of wheat flour arabinoxylan (WAX) and acid debranched arabinoxylan (ADWAX), in the presence and absence of an AXH-d3 α-L-arabinofuranosidase, by several GH10 and GH11 β-xylanases. The structures of the oligosaccharides were characterised by GC-MS and NMR and by hydrolysis by a range of α-L-arabinofuranosidases and β-xylosidase. The AXOS were purified and used to characterise the action patterns of the specific α-L-arabinofuranosidases. These enzymes, in combination with either Cellvibrio mixtus or Neocallimastix patriciarum β -xylanase, were used to produce elevated levels of specific AXOS on hydrolysis of WAX, such as 32-α-L-Araf-(1-4)-β-D-xylobiose (A3X), 23-α-L-Araf-(1-4)-β-D-xylotriose (A2XX), 33-α-L-Araf-(1-4)-β-D-xylotriose (A3XX), 22-α-L-Araf-(1-4)-β-D-xylotriose (XA2X), 32-α-L-Araf (1-4)-β-D-xylotriose (XA3X), 23-α-L-Araf-(1-4)-β-D-xylotetraose (XA2XX), 33-α-L-Araf-(1-4)-β-D-xylotetraose (XA3XX), 23 ,33-di-α-L-Araf-(1-4)-β-D-xylotriose (A2+3XX), 23,33-di-α-L-Araf-(1-4)-β-D-xylotetraose (XA2+3XX), 24,34-di-α-L-Araf-(1-4)-β-D-xylopentaose (XA2+3XXX) and 33,34-di-α-L-Araf-(1-4)-β-D-xylopentaose (XA3A3XX), many of which have not previously been produced in sufficient quantities to allow their use as substrates in further enzymic studies. For A2,3XX, yields of approximately 16% of the starting material (wheat arabinoxylan) have been achieved. Mixtures of the α-L-arabinofuranosidases, with specific action on AXOS, have been combined with β-xylosidase and β-xylanase to obtain an optimal mixture for hydrolysis of arabinoxylan to L-arabinose and D-xylose.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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