The product has been successfully added to your shopping list.

Total and Free Sulfite Assay Kit (Liquid Ready)

Play Training Video

00:02  Introduction & Kit Description
01:20   Theory of Assay Procedure
01:41    Standards & Calibration Curves
01:58    Preparation of the single point standard
03:44    Preparation of the Calibration Curve
06:11    Assay Procedure
10:10    Free Sulphite Calculations

Total and Free Sulfite Assay Kit K-SULPH Scheme
   
Product code: K-SULPH
€143.00

40 assays of each (manual) / 400 assays of each (microplate) / 400 assays of each (auto-analyser)

Prices exclude VAT

Available for shipping

North American customers click here
Content: 40 assays of each (manual) / 400 assays of each (microplate) / 400 assays of each (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: Ambient,
Long term stability: See individual component labels
Stability: > 6 months under recommended storage conditions
Analyte: Free Sulfite, Total Sulfite
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 405,
575
Signal Response: Increase
Linear Range: 0.25 to 20 µg of TSO2 per assay
Limit of Detection: ~ 5.3 mg/L (Total sulfite),
~ 2 mg/L (Free sulfite)
Total Assay Time: ~ 6 min (Total sulfite),
~ 9 min (Free sulfite)
Application examples: Wine, fruit juice, sea food, food stuffs and other materials.
Method recognition: Validated for red and white wines at the Bundesamt für Weinbau, Austria. Used widely in the wine industry

The Sulfite Test Kit for the determination and measurement of total sulfite (sulphite) and free sulfite in wine, beverages, food stuffs and other materials.

This rapid method contains a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.

More sulfite assay kits available.

Scheme-K-SULPH SULPH Megazyme

Advantages
  • ”Ready to use" liquid stable Formulation 
  • Very competitive price (cost per test) 
  • All reagents stable for > 18 months 
  • Very rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats
Publications
Publication

Impact of microoxygenation on Pinot noir wines with different initial phenolic content.

Yang, Y., Deed, R. C., Araujo, L. D. & Kilmartin, P. A. (2021). OENO One, 55(4), 83-100.

Microoxygenation (MOX) is used to improve wine colour and sensory quality; however, limited information is available for Pinot noir wines and wines with different initial phenolic content. In this study, MOX was applied to two Pinot noir wines, with either a low or a high phenolic content, at two doses (0.50 and 2.11 mg/L/day) for 14 days. With the sterile filtration applied, acetaldehyde formation during MOX was very low, supporting the influence of yeast on acetaldehyde production during MOX. The MOX dosage rate did not significantly affect colour development, while the Pinot noir wine with higher phenolics benefited more from MOX, significantly increasing colour intensity and SO2 resistant (polymeric) pigments. However, these changes did not guarantee colour stability, as a final SO2 addition (100 mg/L) largely erased the improvement to colour in all wines. This could be due to the lower acetaldehyde formation, thus less ethyl-bridged stable pigments resistant to SO2 bleaching. MOX also decreased the flavan-3-ols and anthocyanin monomers, which differed between the two Pinot noir wines, reflecting the initial phenolic content. Lastly, MOX generally increased the measured tannin concentration and affected the proportion of tannin subunits, with a decrease in tannin mass conversion and proportion of (-)-epigallocatechin extension units. Some of these changes in phenolic compounds could potentially increase astringency, suggesting that MOX should be applied to Pinot noir and other low phenolic wines with caution.

Hide Abstract
Safety Information
Symbol : GHS07, GHS08
Signal Word : Danger
Hazard Statements : H315, H319, H350
Precautionary Statements : P201, P202, P264, P280, P302+P352, P305+P351+P338, P308+P313, P321, P332+P313, P405, P501
Safety Data Sheet
Customers also viewed
Total Sulfite Assay Kit Enzymatic K-ETSULPH ETSULPH
Total Sulfite Assay Kit (Enzymatic)
€186.00
Total Sulfite Assay Kit K-TSULPH TSULPH
Total Sulfite Assay Kit (Liquid Ready)
€143.00
Pullulanase M2 Bacillus licheniformis E-PULBL
Pullulanase M2 (Bacillus licheniformis)
€165.00
Free Sulfite Assay Kit K-FSULPH FSULPH
Free Sulfite Assay kit (Liquid Ready)
€0.00
D-Lactic Acid D-Lactate Rapid Assay Kit K-DATE DATE
D-Lactic Acid (D-Lactate) (Rapid) Assay Kit
€183.00
D-3-Hydroxybutyric Acid beta-Hydroxybutyrate Assay Kit K-HDBA HDBA
D-3-Hydroxybutyric Acid (β-Hydroxybutyrate) Assay Kit
€171.00
Rapid Integrated Total Dietary Fiber Assay Kit K-RINTDF RINTDF
Rapid Integrated Total Dietary Fiber Assay Kit
€281.00
Acetic Acid Assay Kit ACS Manual Format K-ACET ACET
Acetic Acid Assay Kit (ACS Manual Format)
€164.00