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|Formulation:||In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4|
|Stability:||Minimum 1 year at 4oC. Check vial for details.|
|Synonyms:||alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum)|
|Concentration:||Supplied at ~ 200 U/mL|
|Expression:||Recombinant from Escherichia coli|
Catalyses the reaction:
A phosphate monoester + H2O = an alcohol + phosphate
|Specific Activity:||~ 10 U/mg (37oC, pH 10.4 on p-nitrophenyl phosphate)|
|Unit Definition:||One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37oC.|
|Application examples:||Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA).|
High purity recombinant Alkaline phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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