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Phosphatase (Alkaline) (Escherichia coli)

Product code: E-ALPEC

400 Units

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This product has been discontinued (read more)

Content: 400 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Phosphatase
EC Number:
CAS Number: 9001-78-9
Synonyms: alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Source: Escherichia coli
Molecular Weight: 50,503
Concentration: Supplied at ~ 200 U/mL
Expression: Recombinant from Escherichia coli
Specificity: Catalyses the reaction:
A phosphate monoester + H2O = an alcohol + phosphate
Specific Activity: ~ 10 U/mg (37oC, pH 10.4 on p-nitrophenyl phosphate)
Unit Definition: One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37oC.
Temperature Optima: 37oC
pH Optima: 10.4
Application examples: Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA).

This product has been discontinued (read more).

High purity recombinant Alkaline phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Explore more available analytical enzymes.

Certificate of Analysis
Safety Data Sheet
Data Sheet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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