Phosphatase (Alkaline) (Escherichia coli)
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Phosphatase |
| EC Number: | 3.1.3.1 |
| CAS Number: | 9001-78-9 |
| Synonyms: | alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum) |
| Source: | Escherichia coli |
| Molecular Weight: | 50,503 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: |
Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
| Specific Activity: | ~ 10 U/mg (37oC, pH 10.4 on p-nitrophenyl phosphate) |
| Unit Definition: | One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 10.4 |
| Application examples: | Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA). |
This product has been discontinued (read more).
High purity recombinant Alkaline phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Explore more available analytical enzymes.
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Safety Data Sheet
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