Phosphatase (Alkaline) (Escherichia coli)
Reference code: E-ALPEC
400 Units
This product has been discontinued
Content:
400 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4
Physical Form:
Suspension
Stability:
Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity:
Phosphatase
EC Number:
3.1.3.1
CAS Number:
9001-78-9
Synonyms:
alkaline phosphatase; phosphate-monoester phosphohydrolase (alkaline optimum); Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Source:
Escherichia coli
Molecular Weight:
50,503
Concentration:
Supplied at ~ 200 U/mL
Expression:
Recombinant from Escherichia coli
Specificity:
Catalyses the reaction:
A phosphate monoester + H2O = an alcohol + phosphate
A phosphate monoester + H2O = an alcohol + phosphate
Specific Activity:
~ 10 U/mg (37oC, pH 10.4 on p-nitrophenyl phosphate)
Unit Definition:
One Unit of alkaline phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from p-nitrophenyl phosphate (1 mM), in glycine buffer (95.3 mM), pH 10.4 at 37oC.
Temperature Optima:
37oC
pH Optima:
10.4
Application examples:
Widely used in molecular biology and in enzyme-linked immunosorbent assays (ELISA).
This product has been discontinued (read more).
High purity recombinant Alkaline phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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