Galactazyme Tablets
To choose a chapter, play the video and select the required chapter from the options on the video display.
Chapter 1: Theory of endo-1, 4-Beta-D-Xylanase Assay Procedure
Chapter 2: Buffers & Reagents
Chapter 3: Assay Procedure
200 Tablets
Prices exclude VAT
Available for shipping
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 10 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Galactanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
The T-GLZ-1000T pack size has been discontinued (read more).
High purity dyed and crosslinked Galactazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of endo-1,4-β-D-galactanase. Containing AZCL-Galactan (potato).
Please note the video above shows the protocol for assay of endo-xylanase using xylazyme tablets. The procedure for the assay of endo-1,4-β-galactanase using Galactazyme Tablets is equivalent to this.
View our complete list of enzyme tablet tests.
de Vries, R. P., Pařenicová, L., Hinz, S. W. A., Kester, H. C. M., Beldman, G., Benen, J. A. E. & Visser, J. (2002). European Journal of Biochemistry, 269(20), 4985-4993.
The Aspergillus niger β-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression of galA in A. niger was only detected in the presence of sugar beet pectin, D-galacturonic acid and L-arabinose, suggesting that galA is coregulated with both the pectinolytic genes as well as the arabinanolytic genes. The corresponding enzyme, endogalactanase A (GALA), contains both active site residues identified previously for the Pseudomonas fluorescens β-1,4-endogalactanase. The galA gene was overexpressed to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of arabinogalactans of potato, onion and soy with GALA resulted initially in the release of D-galactotriose and D-galactotetraose, whereas prolonged incubation resulted in D-galactose and D-galactobiose, predominantly. MALDI-TOF analysis revealed the release of L-arabinose substituted D-galactooligosaccharides from soy arabinogalactan. This is the first report of the ability of a β-1,4-endogalactanase to release substituted D-galacto-oligosaccharides. GALA was not active towards D-galacto-oligosaccharides that were substituted with D-glucose at the reducing end.
Hide Abstract| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
