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endo-1,4-β-Xylanase (Aspergillus aculeatus)

endo-1-4-beta-Xylanase Aspergillus aculeatus E-XYLAA
Product code: E-XYLAA
€155.00

3.125 mL; 5,000 Units

Prices exclude VAT

Available for shipping

Content: 3.125 mL; 5,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: endo-1,4-β-Xylanase
EC Number: 3.2.1.8
CAZy Family: GH11
CAS Number: 9025-57-4
Synonyms: endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase
Source: Aspergillus aculeatus
Molecular Weight: 52,000
Concentration: Supplied at ~ 1,600 U/mL
Expression: Purified from Aspergillus aculeatus
Specificity: endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.
Specific Activity: ~ 30 U/mg (40oC, pH 4.5 on wheat arabinoxylan)
Unit Definition: One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents from wheat arabinoxylan per minute under the conditions stated.
Temperature Optima: 80oC
pH Optima: 4.5
Application examples: For use in research.

High purity endo-1,4-β-Xylanase (Aspergillus aculeatus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

View all of our Carbohydrate Active enZYme products.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication
Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside. The development of the substrate and associated assay is discussed here and the relationship between the activity values obtained with the XylX6 assay versus traditional reducing sugar assays and its specificity and reproducibility were thoroughly investigated.

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Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
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