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|Stability:||> 10 years under recommended storage conditions|
|Main Chain Glycosidic Linkage:||β-1,4|
|Substrate For (Enzyme):||endo-Chitinase|
High purity Chitin (shrimp shells) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
This substrate is recommended for the assay of endo-chitinase using reducing sugar methods.
Equivalent to Sigma product Chitin from shrimp (#C9752).
Hoßbach, J., Bußwinkel, F., Kranz, A., Wattjes, J., Cord-Landwehr, S. & Moerschbacher, B. M. (2017). Carbohydrate Polymers, 183, 1-10.
Chitosan is a structurally diverse biopolymer that is commercially derived from chitin by chemical processing, but chitin deacetylases (CDAs) potentially offer a sustainable and more controllable approach allowing the production of chitosans with tailored structures and biological activities. We investigated the CDA from Podospora anserina (PaCDA) which is closely related to Colletotrichum lindemuthianum CDA in the catalytic domain, but unique in having two chitin-binding domains. We produced recombinant PaCDA in Hansenula polymorpha for biochemical characterization and found that the catalytic domain of PaCDA is also functionally similar to C. lindemuthianum CDA, though differing in detail. When studying the enzyme’s mode of action on chitin oligomers by quantitative mass-spectrometric sequencing, we found almost all possible sequences up to full deacetylation but with a clear preference for specific products. Deletion muteins lacking one or both CBDs confirmed their proposed function in supporting the enzymatic conversion of the insoluble substrate colloidal chitin.Hide Abstract