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Chitin (shrimp shells)

Colloidal Chitin (shrimp shells) P-CHITN
Product code: P-CHITN

1 g

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Available for shipping

Content: 1 g
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 1398-61-4
Synonyms: beta-(1,4)-2-Acetamido-2-deoxy-D-glucose; Poly-(1,4)-β-N-acetyl-D-glucosamine
Source: Shrimp shells
Monosaccharides (%): N-Acetyl-Glucosamine
Main Chain Glycosidic Linkage: β-1,4
Substrate For (Enzyme): endo-Chitinase

High purity Chitin (shrimp shells) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

This substrate is recommended for the assay of endo-chitinase using reducing sugar methods.

Equivalent to Sigma product Chitin from shrimp (#C9752).

Certificate of Analysis
Safety Data Sheet
A chitin deacetylase of Podospora anserina has two functional chitin binding domains and a unique mode of action.

Hoßbach, J., Bußwinkel, F., Kranz, A., Wattjes, J., Cord-Landwehr, S. & Moerschbacher, B. M. (2017). Carbohydrate Polymers, 183, 1-10.

Chitosan is a structurally diverse biopolymer that is commercially derived from chitin by chemical processing, but chitin deacetylases (CDAs) potentially offer a sustainable and more controllable approach allowing the production of chitosans with tailored structures and biological activities. We investigated the CDA from Podospora anserina (PaCDA) which is closely related to Colletotrichum lindemuthianum CDA in the catalytic domain, but unique in having two chitin-binding domains. We produced recombinant PaCDA in Hansenula polymorpha for biochemical characterization and found that the catalytic domain of PaCDA is also functionally similar to C. lindemuthianum CDA, though differing in detail. When studying the enzyme’s mode of action on chitin oligomers by quantitative mass-spectrometric sequencing, we found almost all possible sequences up to full deacetylation but with a clear preference for specific products. Deletion muteins lacking one or both CBDs confirmed their proposed function in supporting the enzymatic conversion of the insoluble substrate colloidal chitin.

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Safety Information
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Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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