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α-Fucosidase (Thermotoga maritima)

alpha-Fucosidase Thermostable Thermotoga maritima E-FUCTM
Product code: E-FUCTM
€115.00

10 Units at 25oC

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Available for shipping

Content: 10 Units at 25oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: α-Fucosidase
EC Number: 3.2.1.51
CAZy Family: GH29
CAS Number: 9037-65-4
Synonyms: alpha-L-fucosidase; alpha-L-fucoside fucohydrolase
Source: Thermotoga maritima
Molecular Weight: 54,351
Concentration: Supplied at ~ 5 U/mL
Expression: Recombinant from Thermotoga maritima
Specificity: Hydrolysis of α-L-fucose residues from the non-reducing terminal residues in glycoproteins and oligosaccharides.
Specific Activity: ~ 2.5 U/mg protein (25oC); 
~ 4 U/mg protein (37oC); 
~ 15 U/mg protein (60oC);  
~ 200 U/mg protein (95oC)
Unit Definition: One Unit of α-fucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-fucopyranoside in citrate buffer (50 mM)/phosphate buffer (100 mM), pH 5.0.
Temperature Optima: 95oC
pH Optima: 5
Application examples: For use in glycobiology research.

High purity recombinant α-Fucosidase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Improvement of the transfucosylation activity of α-L-fucosidase from Thermotoga maritima for the synthesis of fucosylated oligosaccharides in the presence of calcium and sodium.

Guzmán-Rodríguez, F., Alatorre-Santamaría, S., Gómez-Ruiz, L., Rodríguez-Serrano, G., García-Garibay, M. & Cruz-Guerrero, A. (2018). Extremophiles, 1-6.

The influence of CaCl2 and NaCl in the hydrolytic activity and the influence of CaCl2 in the synthesis of fucosylated oligosaccharides using α-L-fucosidase from Thermotoga maritima were evaluated. The hydrolytic activity of α-L-fucosidase from Thermotoga maritima displayed a maximum increase of 67% in the presence of 0.8 M NaCl with water activity (aw) of 0.9672 and of 138% in the presence of 1.1 M CaCl2 (aw 0.9581). In addition, the hydrolytic activity was higher when using CaCl2 compared to NaCl at aw of 0.8956, 0.9581 and 0.9672. On the other hand, the effect of CaCl2 in the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor was studied. In these reactions, the presence of 1.1 M CaCl2 favored the rate of transfucosylation, and improved the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 and 146 mM, respectively. CaCl2 did not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in aw and lactose concentration had a synergistic effect favoring the synthesis of fucosylated oligosaccharides.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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