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Alcohol dehydrogenase (Escherichia coli)

Product code: E-ADHEC

1,000 Units

Prices exclude VAT

Available for shipping

Content: 1,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: Dehydrogenase
EC Number:
CAS Number: 9031-72-5
Synonyms: alcohol dehydrogenase; alcohol:NAD+ oxidoreductase
Source: Escherichia coli
Molecular Weight: 38,642
Concentration: Supplied at ~ 320 U/mL
Expression: Recombinant from Escherichia coli
Specificity: Catalyses the reaction:
Primary alcohol + NAD+ = an aldehyde + NADH + H+ 
This enzyme works on primary or secondary alcohols or hemi-acetals with very broad specificity; however the enzyme oxidizes methanol much more poorly than ethanol. 
Specific Activity: > 11 U/mg (25oC, pH 8.5 on ethanol)
Unit Definition: One Unit of alcohol dehydrogenase activity is defined as the amount of enzyme required to oxidise one µmole of ethanol per minute, in the presence of NAD+, in potassium pyrophosphate buffer (86 mM), pH 8.5 at 25oC.
Temperature Optima: 25oC
pH Optima: 8.5
Application examples: Applications for the measurement of ethanol in the food, wine and beverage and pharmaceutical industries.

High purity recombinant Alcohol dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

View more of our enzyme products for analytical methods.

Certificate of Analysis
Safety Data Sheet
Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
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