The product has been successfully added to your shopping list.

1,3-β-Glucazyme Tablets

Play Training Video
Analysis of enzymes activity using carbohydrase tablet testing

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Theory of endo-1, 4-Beta-D-Xylanase Assay Procedure
Chapter 2: Buffers & Reagents
Chapter 3: Assay Procedure
1-3-beta-Glucazyme Tablets T-PAZ-200T T-PAZ
   
Product code: T-PAZ-200T
€249.00

200 Tablets

Prices exclude VAT

Available for shipping

Content: 200 Tablets
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Solid
Stability: > 10 years under recommended storage conditions
Substrate For (Enzyme): endo-1,3-β-Glucanase
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 590
Reproducibility (%): ~ 5%

High purity dyed and crosslinked 1,3-β-Glucazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,3-β-D-glucanase (laminarinase). Containing AZCL-Pachyman; a 1,3-β-D-glucan.

Please note the video above shows the protocol for assay of endo-xylanase using xylazyme tablets. The procedure for the assay of endo-1,3-β-glucanase using 1,3-β-Glucazyme Tablets is equivalent to this.

Documents
Certificate of Analysis
Safety Data Sheet
FAQs Booklet
Publications
Publication
Lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence.

Sakamoto, Y., Watanabe, H., Nagai, M., Nakade, K., Takahashi, M. & Sato, T. (2006). Plant Physiology, 141(2), 793-801.

Lentinan is an antitumor product that is purified from fresh Lentinula edodes fruiting bodies. It is a cell wall component, comprising β-1,3-glucan with β-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. In this study, we used N-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (TL) protein in L. edodes. The cDNA clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and comparison of the two indicated that tlg1 contains 12 introns. The tlg1 gene product (TLG1) was predicted to comprise 240 amino acids, with a molecular mass of 25 kD and isoelectric point value of 3.5. The putative amino acid sequence exhibits approximately 40% identity with plant TL proteins, and a fungal genome database search revealed that these TL proteins are conserved in many fungi including the basidiomycota and ascomycota. Transcription of tlg1 was not detected in vegetative mycelium or young and fresh mushrooms. However, transcription increased following harvest. Western-blot analysis demonstrated a rise in TLG1 levels following harvest and spore diffusion. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited β-1,3-glucanase activity and, when purified from the L. edodes fruiting body, demonstrated lentinan degrading activity. Thus, we suggest that TLG1 is involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion.

Hide Abstract
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Customers also viewed