Phosphatase (Acid) (Escherichia coli)
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Phosphatase |
| EC Number: | 3.1.3.2 |
| CAS Number: | 9001-77-8 |
| Synonyms: | acid phosphatase; phosphate-monoester phosphohydrolase (acid optimum); orthophosphoric-monoester phosphohydrolase (acid optimum) |
| Source: | Escherichia coli |
| Molecular Weight: | 24,667 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: |
Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
| Specific Activity: | ~ 14 U/mg (40oC, pH 5.0 on 4-Nitrophenyl phosphate) |
| Unit Definition: | One Unit of acid phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl phosphate (4 mM) in sodium acetate buffer (20 mM), pH 5.0 at 40oC. |
| Temperature Optima: | 45oC |
| pH Optima: | 5 |
| Application examples: | Applications in research including molecular biology and biocatalysis. |
This product has been discontinued (read more).
High purity recombinant Acid phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
We offer other enzyme products for analytical and research applications.
Safety Information
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P284, P304+P340, P342+P311, P501 |
Safety Data Sheet
