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Phosphatase (Acid) (Escherichia coli)
Product code:
E-ACPEC
€127.00
400 Units
Prices exclude VAT
Available for shipping
400 Units
Prices exclude VAT
Available for shipping
Content: | 400 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | Phosphatase |
EC Number: | 3.1.3.2 |
CAS Number: | 9001-77-8 |
Synonyms: | acid phosphatase; phosphate-monoester phosphohydrolase (acid optimum); orthophosphoric-monoester phosphohydrolase (acid optimum) |
Source: | Escherichia coli |
Molecular Weight: | 24,667 |
Concentration: | Supplied at ~ 200 U/mL |
Expression: | Recombinant from Escherichia coli |
Specificity: |
Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
Specific Activity: | ~ 14 U/mg (40oC, pH 5.0 on 4-Nitrophenyl phosphate) |
Unit Definition: | One Unit of acid phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl phosphate (4 mM) in sodium acetate buffer (20 mM), pH 5.0 at 40oC. |
Temperature Optima: | 45oC |
pH Optima: | 5 |
Application examples: | Applications in research including molecular biology and biocatalysis. |
High purity recombinant Acid phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
We offer other enzyme products for analytical and research applications.