Phosphatase (Acid) (Escherichia coli)
Reference code: E-ACPEC
400 Units
This product has been discontinued
Content:
400 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4
Physical Form:
Suspension
Stability:
Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity:
Phosphatase
EC Number:
3.1.3.2
CAS Number:
9001-77-8
Synonyms:
acid phosphatase; phosphate-monoester phosphohydrolase (acid optimum); orthophosphoric-monoester phosphohydrolase (acid optimum)
Source:
Escherichia coli
Molecular Weight:
24,667
Concentration:
Supplied at ~ 200 U/mL
Expression:
Recombinant from Escherichia coli
Specificity:
Catalyses the reaction:
A phosphate monoester + H2O = an alcohol + phosphate
A phosphate monoester + H2O = an alcohol + phosphate
Specific Activity:
~ 14 U/mg (40oC, pH 5.0 on 4-Nitrophenyl phosphate)
Unit Definition:
One Unit of acid phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl phosphate (4 mM) in sodium acetate buffer (20 mM), pH 5.0 at 40oC.
Temperature Optima:
45oC
pH Optima:
5
Application examples:
Applications in research including molecular biology and biocatalysis.
This product has been discontinued (read more).
High purity recombinant Acid phosphatase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
We offer other enzyme products for analytical and research applications.
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