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Lactose/Sucrose/D-Glucose Assay Kit

Lactose Sucrose D-Glucose Assay Kit K-LACSU
Product code: K-LACSU
€234.00

100 assays of each per kit

Prices exclude VAT

Available for shipping

Content: 100 assays of each per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: D-Glucose, Lactose, Sucrose
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 510
Signal Response: Increase
Limit of Detection: 100 mg/L
Reaction Time (min): ~ 60 min
Application examples: Flours, beverages, dairy products, milk, foodstuffs containing milk, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Used and accepted in food analysis

The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.


Advantages
  • Very competitive price (cost per test) 
  • All reagents stable for > 12 months after preparation 
  • Simple format 
  • Very specific 
  • Rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included
Documents
Certificate of Analysis
Safety Data Sheet
FAQs Booklet Data Calculator
Publications
Publication
Construction of a food-grade cell surface display system for Lactobacillus casei.

Qin, J., Wang, X., Kong, J., Ma, C. & Xu, P. (2014). Microbiological Research, 169(9-10), 733-740.

In this study, a food-grade cell surface display host/vector system for Lactobacillus casei was constructed. The food-grade host L. casei Q-5 was a lactose-deficient derivative of L. casei ATCC 334 obtained by plasmid elimination. The food-grade cell surface display vector was constructed based on safe DNA elements from lactic acid bacteria containing the following: pSH71 replicon from Lactococcus lactis, lactose metabolism genes from L. casei ATCC 334 as complementation markers, and surface layer protein gene from Lactobacillus acidophilus ATCC 4356 for cell surface display. The feasibility of the new host/vector system was verified by the expression of green fluorescent protein (GFP) on L. casei. Laser scanning confocal microscopy and immunofluorescence analysis using anti-GFP antibody confirmed that GFP was anchored on the surface of the recombinant cells. The stability of recombinant L. casei cells in artificial gastrointestinal conditions was verified, which is beneficial for oral vaccination applications. These results indicate that the food-grade host/vector system can be an excellent antigen delivery vehicle in oral vaccine construction.

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Publication
Polymerization of lactose by twin-screw extrusion to produce indigestible oligosaccharides.

Tremaine, A. J., Reid, E. M., Tyl, C. E. & Schoenfuss, T. C. (2014). International Dairy Journal, 36(1), 74-81.

Soluble dietary fiber is a growing food ingredient market. Twin-screw extrusion of lactose with an acid catalyst has been reported as a way to polymerize lactose to oligomers which are indigestible and analyze as dietary fiber. Lactose was dry blended with citric acid at two different concentrations, 1 and 2%. Glucose was added to raw mixes at 0, 10, or 20% (w/w). Samples with 2% citric acid yielded a higher concentration of indigestible oligosaccharides (52.3–59.8%, w/w) than 1% citric acid samples (37.1–49.9%). Glucose did not affect the yield. Glucose addition was beneficial and reduced the motor torque and specific mechanical energy of the extruder, and extruded products were lighter in color. The generated oligomers had a degree of polymerization that ranged from 3 to 5, as determined by mass spectrometry. Testing if the oligomers confer a beneficial effect (to be classified as dietary fiber), is still required.

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Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H314, H315, H319, H334
Precautionary Statements : P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P342+P311, P501
Safety Data Sheet
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