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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||> 4 years at 4oC|
|Synonyms:||alpha-mannosidase; alpha-D-mannoside mannohydrolase|
|Concentration:||Supplied at ~ 100 U/mL|
|Expression:||Recombinant from Bacteroides thetaiotaomicron|
|Specificity:||Hydrolysis of terminal non-reducing α-D-mannose in α-(1-3), α-(1-4) and α-(1-6)-D-mannosides.|
|Specific Activity:||~ 1 U/mg protein (40oC, pH 6.5 on pNP-α-D-mannopyranoside)|
|Unit Definition:||One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-mannopyranoside (5 mM) in MES buffer (100 mM) and CaCl2 (2.5 mM) at pH 6.5 at 40oC.|
|Application examples:||For use in research.|
High purity α-D-Mannosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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