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4-Nitrophenyl-β-D-galacturonide

4-Nitrophenyl-beta-D-galacturonide O-PNPBGALA
Product code: O-PNPBGALA
€170.00

20 mg

Prices exclude VAT

Available for shipping

Content: 20 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Solid
Stability: > 5 years under recommended storage conditions
CAS Number: 39031-76-0
Molecular Formula: C12H13NO9
Molecular Weight: 315.2
Purity: > 95%
Substrate For (Enzyme): β-Galacturonidase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-β-D-galacturonide for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of β-galacturonidase activity.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Molecular and biochemical analysis of two β-galactosidases from Bifidobacterium infantis HL96.

Hung, M., N., Xia, Z., Hu, N-T. & Lee, B. H. (2001). Applied and Environmental Microbiology, 67(9), 4256-4263.

Two genes encoding β-galactosidase isoenzymes, β-galI and β-galIII, from Bifidobacterium infantis HL96 were revealed on 3.6- and 2.4-kb DNA fragments, respectively, by nucleotide sequence analysis of the two fragments. β-galI (3,069 bp) encodes a 1,022-amino-acid (aa) polypeptide with a predicted molecular mass of 113 kDa. A putative ribosome binding site and a promoter sequence were recognized at the 5′ flanking region of β-galI. Further upstream a partial sequence of an open reading frame revealed a putative lactose permease gene transcribing divergently from β-galI. The β-galIII gene (2,076 bp) encodes a 691-aa polypeptide with a calculated molecular mass of 76 kDa. A rho-independent transcription terminator-like sequence was found 25 bp downstream of the termination codon. The amino acid sequences of β-GalI and β-GalIII are homologous to those found in the LacZ and the LacG families, respectively. The acid-base, nucleophilic, and substrate recognition sites conserved in the LacZ family were found in β-GalI, and a possible acid-base site proposed for the LacG family was located in β-GalIII, which featured a glutamate at residue 160. The coding regions of the β-galI and β-galIII genes were each cloned downstream of a T7 promoter for overexpression in Escherichia coli. The molecular masses of the overexpressed proteins, as estimated by polyacrylamide gel electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, agree with their predicted molecular weights. β-GalI and β-GalIII were specific for β-D-anomer-linked galactoside substrates. Both are more active in response to ONPG (o-nitrophenyl-β-D-galactopyranoside) than in response to lactose, particularly β-GalIII. The galacto-oligosaccharide yield in the reaction catalyzed by β-GalI at 37°C in 20% (wt/vol) lactose solution was 130 mg/ml, which is more than six times higher than the maximum yield obtained with β-GalIII. The structure of the major trisaccharide produced by β-GalI catalysis was characterized as O-β-D-galactopyranosyl-(1-3)- O-β-D-galactopyranosyl-(1-4)-D-glucopyranose (3′-galactosyl-lactose).

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Publication
The relationship between glucuronidase and galacturonidase activity in the limpet and in mammalian tissues.

Marsh, C. A. & Levvy, G. A. (1958). Biochemical Journal, 68(4), 610-617.

In the first systematic study of the enzyme β-glucuronidase, Masamune showed that oxkidney preparations did not hydrolyse (-)-menthyl α-D-glucuronide, and this compound was also not hydrolysed by mouse-liver β-glucuronidase and by a snail preparation rich in β-glucuronidase and in other simple glycosidases. Nevertheless, although it is not so ubiquitous as the β-glucuronide residue, there is evidence for a naturally occurring α-conjugated glucuronic acid, for example in wheat straw. It has been inferred that uridine diphosphoglucuronic acid also has the α-configuration.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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