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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||Minimum 1 year at 4oC. Check vial for details.|
|Enzyme Activity:||Xanthan Lyase|
|Synonyms:||xanthan lyase; xanthan lyase|
|Concentration:||Supplied at ~ 10,000 U/mL|
|Expression:||Recombinant from Bacillus sp.|
|Specificity:||β-elimination cleavage of the terminal β-D-mannosyl-β-D-1,4-glucuronosyl linkage of the side-chain of xanthan.|
|Specific Activity:||~ 1,800 U/mg (40oC, pH 6.0 on xanthan gum)|
|Unit Definition:||One Unit of xanthan lyase activity is defined as the amount of enzyme required to release an increase in absorbance of 1.0 per minute on xanthan gum (5 mg/mL) in HEPES buffer (100 mM), pH 6.0 at 40oC.|
|Application examples:||Applications in biotechnology and the analysis and measurement of xanthan.|
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification.
de Sousa, B. F. S., Castellane, T. C. L., Campanharo, J. C. & de Macedo Lemos, E. G. (2019). International Journal of Biological Macromolecules, 136, 424-435.
Enzymes can be very useful on exopolysaccharides (EPS) research, can be used at elucidation and also to modify the polysaccharides' structure in order to alter their physical properties. Thus, the reduction of the molecular mass could increase applications of these biopolymers. Therefore, the EPS production of different rhizobia and the action of xanthan lyase on its structures were evaluated. The strains produced significant amounts of EPS, and it was noticed that are heteropolysaccharides, composed galactose and glucose. Both EPS and xanthan were modified on β-glycosidic bonds, the mannose was removed of xanthan had but the EPS was affected in the CO stretching vibration, where the glucuronic acid removed from of your structure. The ester/carboxylic acid portions affected functional groups of the acetate/succinate, methyl carbons of the O-acetyl and pyruvate methyl groups in addition to affect the carbons the main pyranoid. The Resistance to temperature increase of the EPS was observed, made possible by the activity of the lyase. EPS has the ability to form stable gels at higher temperatures and anionic feature can be used on solubilization and controlled release of substances. Modified EPS knowledge will presently facilitate future investigations relating the structure of the rhizobia polysaccharide against rheological properties.Hide Abstract