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Hydrogen Peroxide Assay Kit (Megaplex Red)

Product code: K-MRH2O2
€250.00

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

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Content: 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Hydrogen Peroxide
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance, Fluorescence
Wavelength (nm): (Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570
Signal Response: Increase
Linear Range: (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay)
(Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay)
Limit of Detection: (Fluorescence) 0.0034 ug/mL
(Absorbance)
 0.25 ug/mL
Reproducibility (%): (Fluorescence) ~ 8%
(Absorbance) ~ 8%
Reaction Time (min): (Fluorescence) 30 min
(Absorbance)
 
15 min
Application examples: Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Display all assay test kits available.

Fluorometric/UV method for the determination of H2O2 content in a variety of samples.

Scheme-K-MRH2O2 MRH2O2 Megazyme

Advantages
  • Simple format
  • Very competitive price (cost per test)
  • Ability to run in fluorescence or absorbance mode
  • User friendly - Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Suitable for manual, microplate and auto-analyser formats
  • All reagents stable for > 2 years
Documents
Certificate of Analysis
Safety Data Sheet
Assay Protocol Data Calculator
Safety Information
Symbol : GHS06
Signal Word : Danger
Hazard Statements : H301
Precautionary Statements : P264, P270, P301+P310, P321, P330
Safety Data Sheet
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