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Product code: E-SIALST
€0.00
250 Units
Prices exclude VAT
This product has been discontinued (read more)
Content: | 250 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | α-Sialidase |
EC Number: | 3.2.1.18 |
CAZy Family: | GH33 |
CAS Number: | 9001-67-6 |
Synonyms: | exo-alpha-sialidase; acetylneuraminyl hydrolase |
Source: | Salmonella typhimurium |
Molecular Weight: | 42,900 |
Concentration: | Supplied at ~ 2,500 U/mL |
Expression: | Recombinant from Salmonella typhimurium |
Specificity: | Hydrolysis of unbranched, non-reducing terminal α-2,3-linked >> α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates. |
Specific Activity: | ~ 750 U/mg (37oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid) |
Unit Definition: | One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37oC. |
Temperature Optima: | 37oC |
pH Optima: | 7 |
Application examples: | Applications in clinical chemistry, glycobiology and carbohydrate research. |
This product has been discontinued (read more).
High purity recombinant exo-α-sialidase (Salmonella typhimurium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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