endo-1,4-β-Xylanase (Aeromonas punctata)
40 Units
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| Content: | 40 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | endo-1,4-β-Xylanase |
| EC Number: | 3.2.1.8 |
| CAZy Family: | GH10 |
| CAS Number: | 9025-57-4 |
| Synonyms: | endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase |
| Source: | Aeromonas punctata |
| Molecular Weight: | 39,400 |
| Concentration: | Supplied at ~ 40 U/mL |
| Expression: | Recombinant from Aeromonas punctata |
| Specificity: | endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans. |
| Specific Activity: | ~ 5 U/mg (40oC, pH 6.5 on wheat arabinoxylan) |
| Unit Definition: | One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium phosphate buffer (50 mM), pH 6.5. |
| Temperature Optima: | 50oC |
| pH Optima: | 6.5 |
| Application examples: | Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries. |
High purity recombinant endo-1,4-β-Xylanase (Aeromonas punctata) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.
endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside. The development of the substrate and associated assay is discussed here and the relationship between the activity values obtained with the XylX6 assay versus traditional reducing sugar assays and its specificity and reproducibility were thoroughly investigated.
Hide Abstract| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
