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endo-1,4-β-Xylanase (Aeromonas punctata)

Product code: E-XYNAP
€165.00

40 Units

Prices exclude VAT

Available for shipping

Content: 40 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: endo-1,4-β-Xylanase
EC Number: 3.2.1.8
CAZy Family: GH10
CAS Number: 9025-57-4
Synonyms: endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase
Source: Aeromonas punctata
Molecular Weight: 39,400
Concentration: Supplied at ~ 40 U/mL
Expression: Recombinant from Aeromonas punctata
Specificity: endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.
Specific Activity: ~ 5 U/mg (40oC, pH 6.5 on wheat arabinoxylan)
Unit Definition: One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium phosphate buffer (50 mM), pH 6.5.
Temperature Optima: 50oC
pH Optima: 6.5
Application examples: Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

High purity recombinant endo-1,4-β-Xylanase (Aeromonas punctata) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Carbohydrate Active enZYmes products available.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication
Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside. The development of the substrate and associated assay is discussed here and the relationship between the activity values obtained with the XylX6 assay versus traditional reducing sugar assays and its specificity and reproducibility were thoroughly investigated.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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