Prices exclude VAT
Available for shipping
|Content:||5 g or 10 g|
|Stability:||> 5 years under recommended storage conditions|
High purity Calcofluor Fluorescent Stain for use in the measurement of 1,3-1,4-β-glucan in wort and beer.
View all available cofactors and stains.
Data booklets for each pack size are located in the Documents tab.
Tarchevsky, I. A., Ageeva, M. V., Petrova, N. V., Akulov, A. N. & Egorova, A. M. (2017). Applied Biochemistry and Microbiology, 53(5), 568-572.
Chromatography and histochemical analysis of soluble phenolic compounds demonstrated their higher content in the roots of cycloheximide-treated pea plants. These substances accumulated together with lignin in the endodermis and xylem cells of conducting bundles. This finding confirms the antipathogenic cycloheximide effect based on the previous results of proteome analysis.Hide Abstract
Pendrak, M. L. & Roberts, D. D. (2007). Medical Mycology, 45(1), 61-71.
Hemoglobin is an abundant protein in the host vascular compartment and a source of iron, heme, and amino acids for many pathogens. The human fungal pathogen Candida albicans uses hemoglobin as an iron source as well as a signaling molecule to alter gene expression and induce adhesion to several extracellular matrix proteins. We now report that hemoglobin can promote true hyphal morphogenesis. Hemoglobin added to yeast cells at 37°C rapidly induced expression of the hypha-specific genes HWP1 and ECE1 coincident with the pattern of hyphal development. A synthetic medium buffered with phosphate at pH 7.2 and containing physiological glucose (5 mM) and low ammonium ion (0.1 mM) was optimal for the response to hemoglobin. High glucose (110 mM), high ammonium ion (20 mM), and 0.1 mM glutamine were all inhibitory. Heme, free globin, or immobilized hemoglobin could not replicate the activity of hemoglobin to induce germ tubes or hypha-specific gene expression at 37°C under optimized conditions. This implicates the previously described Hb-signaling receptor in hyphal formation. This response was also dependent upon the presence of the morphogenesis regulator Efg1p, but the MAP-kinase specific transcription factor Cph1p was not required. These data define a role for the host-factor hemoglobin in Efg1p-dependent hyphal development.Hide Abstract