The product has been successfully added to your shopping list.

Aldopentauronic acid (internal substitution, borohydride reduced)

Aldopentauronic acid internal substitution borohydride reduced O-XUXXR
Product code: O-XUXXR
€149.00

50 mg

Prices exclude VAT

Available for shipping

Content: 50 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: Not Applicable
Synonyms: 23-(4-O-Methyl-D-glucuronyl)-α-D-xylotetraitol
Molecular Formula: C27H46O23
Molecular Weight: 738.6
Purity: > 50%
Substrate For (Enzyme): endo-1,4-β-Xylanase, endo-Xylanase

23-(4-O-methyl-D-glucuronyl)-α-D-xylotetraitol (XUXX-R) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Prepared by the controlled enzymatic hydrolysis of 4-O-methyl-glucuronoxylan, followed by chromatographic removal of neutral oligomers and borohydride reduction. Note that an unidentified aldouronic acid (suspected to be a higher oligomer) is also present along with the title compound in ratio of approximately 1:1. The unidentified impurity is not hydrolysed by GH67 or GH115 α-glucuronidases or GH43 β-xylosidase. XUXX-R is a selective substrate for GH115 α-glucuronidases and is not hydrolysed by the GH67 family.

Borohydride reduced oligosaccharides are particularly useful as substrates in reducing sugar assays (e.g. Nelson Somogyi) as they do not give rise to the large background / blank value observed for the analogous non-reduced native oligosaccharides.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Identification, heterologous expression and characterization of a novel glycoside hydrolase family 30 xylanase from the fungus Penicillium purpurogenum.

Espinoza, K. & Eyzaguirre, J. (2018). Carbohydrate Research, 468, 45-50.

Penicillium purpurogenum grows on a variety of natural carbon sources and secretes to the medium a large number of enzymes that degrade the polysaccharides present in lignocellulose. In this work, the gene coding for a novel xylanase (XynC) belonging to family 30 of the glycoside hydrolases (GH), has been identified in the genome of the fungus. The enzyme has been expressed in Pichia pastoris and characterized. The mature XynC has 454 amino acid residues and a calculated molecular weight of 49 240. The purified protein shows a molecular weight of 67 000, and it is partially deglycosylated using EndoH. Its pH optimum is in the range of 3-5, and the optimal temperature is 45°C. It is active on both arabinoxylan and glucuronoxylan, similarly to other fungal GH 30 xylanases. It liberates a set of oligosaccharides, which have been detected by thin-layer chromatography, thus indicating that it is an endo-acting xylanase. It hydrolyzes xylooligosaccharides, releasing mainly xylobiose, in contrast to other fungal GH family 30 enzymes which generate chiefly xylose. Highest sequence identity to a characterized family 30 xylanase is found with the enzyme from the fungus Bispora sp (53%). This is the first GH 30 xylanase described from a Penicillium.

Hide Abstract
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Customers also viewed
NEW
Xylan Birchwood partially acetylated P-ACXYL
Xylan (Birchwood, partially acetylated)
€120.00
23-4-O-Methyl-α-D-Glucuronosyl-xylotetraose O-XUXX
23-(4-O-Methyl-α-D-Glucuronyl)-xylotetraose
€166.00
22-4-O-Methyl-α-D-Glucuronosyl-xylotriose O-XUX
22-(4-O-Methyl-α-D-Glucuronyl)-xylotriose
€146.00
Rhamnogalacturonan Soy Bean P-RHAGN
Rhamnogalacturonan (Soy Bean)
€129.00
Rhamnogalacturonan I Potato P-RHAM1
Rhamnogalacturonan I (Potato)
€129.00
Pullulan NaBH4 reduced P-PULLBH
Pullulan (borohydride reduced)
€156.00
Pullulan P-PULLN
Pullulan
€142.00
Pectic Galactan Potato P-PGAPT
Pectic Galactan (Potato)
€119.00