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|Content:||20 g or 100 g|
|Storage Temperature:||Below -10oC|
|Stability:||> 5 years under recommended storage conditions|
|Synonyms:||Adenosine 5’-triphosphate, disodium salt (ATP, 5’-ATP, ATP-Na2)|
|Molecular Weight:||551.1 (anhydrous disodium salt)|
C-ATP-20G: ≥ 99%
C-ATP-100G: ≥ 99%
High purity Adenosine 5’-triphosphate (ATP), Disodium Salt, for use as a cofactor for research, biochemical enzyme assays and in vitro diagnostic analysis.
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Data booklets for each pack size are located in the Documents tab.
Determination of Free and Total Myo-Inositol in Infant Formula and Adult/Pediatric Nutritional Formula by High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection.
Ellingson, D., Pritchard, T., Foy, P., King, K., Mitchell, B., Austad, J., Winters, D., Sullivan, D. & Dowell, D. (2013). Journal of AOAC International, 96(5), 1068-1072.
Determination of Free and Total Myo-Inositol in Infant Formula and Adult/Pediatric Nutritional Formula by High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Including a Novel Total Extraction Using Microwave-Assisted Acid Hydrolysis and Enzymatic Treatment: First Action 2012.12. After an assessment of data generated from a single-laboratory validation study published in J.AOAC Int. 95, 1469–1478 (2012), a method for determining total myo-inositol in infant formula and adult/pediatric nutritional formula by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), including extraction by using microwave-assisted acid hydrolysis and enzymatic treatment was presented for consideration by AOAC during the AOAC Annual Meeting held in Las Vegas, NV, from September 30 to October 3, 2012. The Expert Review Panel on Infant Formula and Adult Nutritionals concluded that the method met the criteria set by the standard method performance requirements (SMPRs) for the determination of free myo-inositol and approved the method as AOAC Official First Action. The method also determines total myo-inositol, but includes bound sources that the SMPRs exclude. The method involves using HPAEC-PAD for free myo-inositol and a total myo-inositol determination by two different techniques. The first technique uses the conventional acid hydrolysis with 6 h incubation in an autoclave. The second uses a microwave-assisted acid hydrolysis with enzymatic treatment that decreases the extraction time.Hide Abstract
Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography.
Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.
An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0 across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4, respectively. Overall average recovery was 99.4 (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106 (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages.Hide Abstract