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Acetyl-CoA synthetase (Bacillus subtilis)
Product code:
E-ACSBS
€208.00
250 Units
Prices exclude VAT
Available for shipping
| Content: | 250 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Other Activities |
| EC Number: | 6.2.1.1 |
| CAS Number: | 9012-31-1 |
| Synonyms: | acetate-CoA ligase; acetate:CoA ligase (AMP-forming) |
| Source: | Bacillus subtilis |
| Molecular Weight: | 65,975 |
| Concentration: | Supplied at ~ 125 U/mL |
| Expression: | Recombinant from Bacillus Subtilis |
| Specificity: |
Catalyses the reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA |
| Specific Activity: |
~ 7.6 U/mg (37oC, pH 7.5 on acetic acid); ~ 39 U/mg (37oC, pH 8.4 on acetic acid); ~ 7.0 U/mg (25oC, pH 7.5 on acetic acid) |
| Unit Definition: | One Unit of acetyl-CoA synthetase activity is defined as the amount of enzyme required to produce one µmole of acetyl coenzyme A from acetic acid, ATP and coenzyme A per min in the presence of NAD+ in TEA buffer (128 mM), pH 8.4 at 25oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 8.4 |
| Application examples: | Applications in the analysis and measurement of acetic acid. |
High purity recombinant Acetyl-CoA synthetase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Safety Data Sheet
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