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Acetyl-CoA synthetase (Bacillus subtilis)

Acetyl-CoA synthetase Bacillus subtilis E-ACSBS
Product code: E-ACSBS

250 Units

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Content: 250 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Other Activities
EC Number:
CAS Number: 9012-31-1
Synonyms: acetate-CoA ligase; acetate:CoA ligase (AMP-forming)
Source: Bacillus subtilis
Molecular Weight: 65,975
Concentration: Supplied at ~ 125 U/mL
Expression: Recombinant from Bacillus Subtilis
Specificity: Catalyses the reaction:
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Specific Activity: ~ 7.6 U/mg (37oC, pH 7.5 on acetic acid); 
~ 39 U/mg (37oC, pH 8.4 on acetic acid);
~ 7.0 U/mg (25oC, pH 7.5 on acetic acid)
Unit Definition: One Unit of acetyl-CoA synthetase activity is defined as the amount of enzyme required to produce one µmole of acetyl coenzyme A from acetic acid, ATP and coenzyme A per min in the presence of NAD+ in TEA buffer (128 mM), pH 8.4 at 25oC.
Temperature Optima: 37oC
pH Optima: 8.4
Application examples: Applications in the analysis and measurement of acetic acid.

High purity recombinant Acetyl-CoA synthetase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Certificate of Analysis
Safety Data Sheet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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