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Acetyl-CoA synthetase (Bacillus subtilis)

Product code: E-ACSBS

250 Units

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Available for shipping

Content: 250 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Other Activities
EC Number:
CAS Number: 9012-31-1
Synonyms: acetate-CoA ligase; acetate:CoA ligase (AMP-forming)
Source: Bacillus subtilis
Molecular Weight: 65,975
Concentration: Supplied at ~ 125 U/mL
Expression: Recombinant from Bacillus Subtilis
Specificity: Catalyses the reaction:
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Specific Activity: ~ 7.6 U/mg (37oC, pH 7.5 on acetic acid); 
~ 39 U/mg (37oC, pH 8.4 on acetic acid);
~ 7.0 U/mg (25oC, pH 7.5 on acetic acid)
Unit Definition: One Unit of acetyl-CoA synthetase activity is defined as the amount of enzyme required to produce one µmole of acetyl coenzyme A from acetic acid, ATP and coenzyme A per min in the presence of NAD+ in TEA buffer (128 mM), pH 8.4 at 25oC.
Temperature Optima: 37oC
pH Optima: 8.4
Application examples: Applications in the analysis and measurement of acetic acid.

High purity recombinant Acetyl-CoA synthetase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

See our complete list of analytical enzymes.

Certificate of Analysis
Safety Data Sheet
Assay Protocol
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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