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63-alpha-D-maltotriosyl-maltotriitol O-MTMTRD
Product code: O-MTMTRD

100 mg

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Content: 100 mg
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 107123-59-1
Synonyms: Borohydride reduced 63-α-D-maltotriosyl-maltotriose
Molecular Formula: C36H64O31
Molecular Weight: 992.9
Purity: > 90%
Substrate For (Enzyme): Amyloglucosidase, Isopullulanase, Neopullulanase, Pullulanase/Limit-Dextrinase

High purity 63-α-D-maltotriosyl-maltotriitol for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as a substrate for the characeterisation of pullulanase or limit dextrinase enzymes using a reducing sugar method (e.g. Nelson-Somogyi). The borohydride reduced analogue is used in place of 63-α-D-maltotriosyl-maltotriose (Cat. No. O-MTMT), because being a reducing sugar itself, the native oligosaccharide generates a large ‘blank’ value in this assay.

Certificate of Analysis
Safety Data Sheet
Megazyme publication
Colourimetric and fluorometric substrates for measurement of pullulanase activity.

McCleary, B. V., Mangan, D., McKie, V., Cornaggia, C., Ivory, R. & Rooney, E. (2014). Carbohydrate Research, 393, 60-69.

Specific and highly sensitive colourimetric and fluorometric substrate mixtures have been prepared for the measurement of pullulanase and limit-dextrinase activity and assays employing these substrates have been developed. These mixtures comprise thermostable α- and β-glucosidases and either 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-maltotriosyl (1-6) α-maltotrioside (BzCNPG3G3, 1) as a colourimetric substrate or 4,6-O-benzylidene-4-methylumbelliferyl-β-maltotriosyl (1-6) α-maltotrioside (BzMUG3G3, 2) as a fluorometric substrate. Hydrolysis of substrates 1 and 2 by exo-acting enzymes such as amyloglucosidase, β-amylase and α-glucosidase is prevented by the presence of the 4,6-O-benzylidene group on the non-reducing end D-glucosyl residue. The substrates are not hydrolysed by any α-amylases studied, (including those from Aspergillus niger and porcine pancreas) and are resistant to hydrolysis by Pseudomonas sp. isoamylase. On hydrolysis by pullulanase, the 2-chloro-4-nitrophenyl-β-maltotrioside (3) or 4-methylumbelliferyl-β-maltotrioside (4) liberated is immediately hydrolysed to D-glucose and 2-chloro-4-nitrophenol or 4-methylumbelliferone. The reaction is terminated by the addition of a weak alkaline solution leading to the formation of phenolate ions in solution whose concentration can be determined using either spectrophotometric or fluorometric analysis. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation.

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Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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