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1,4-β-D-Cellohexaitol (borohydride reduced)

1,4-beta-D-Cellohexaitol borohydride reduced cellohexaose O-CHERD
Product code: O-CHERD
€142.00

10 mg

Prices exclude VAT

Available for shipping

Content: 10 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 61425-46-5
Molecular Formula: C36H64O31
Molecular Weight: 992.9
Purity: > 90%
Substrate For (Enzyme): endo-Cellulase

High purity 1,4-β-D-Cellohexaitol (borohydride reduced) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Hexasaccharide from hydrolysis of cellulose and borohydride reduced. 

Borohydride reduced oligosaccharides are particularly useful as substrates in reducing sugar assays (e.g. Nelson Somogyi) as they do not give rise to the large background / blank value observed for the analogous non-reduced native oligosaccharides.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication

Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research.

Pedersen, H. L., Fangel, J. U., McCleary, B., Ruzanski, C., Rydahl, M. G., Ralet, M. C., Farkas, V., Von Schantz, L., Marcus, S. E., Andersen, M.C. F., Field, R., Ohlin, M., Knox, J. P., Clausen, M. H. & Willats, W. G. T. (2012). Journal of Biological Chemistry, 287(47), 39429-39438.

Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts of analytes. Nucleotide microarrays are well established in plant research, but carbohydrate microarrays are much less established, and one reason for this is a lack of suitable glycans with which to populate arrays. Polysaccharide microarrays are relatively easy to produce because of the ease of immobilizing large polymers noncovalently onto a variety of microarray surfaces, but they lack analytical resolution because polysaccharides often contain multiple distinct carbohydrate substructures. Microarrays of defined oligosaccharides potentially overcome this problem but are harder to produce because oligosaccharides usually require coupling prior to immobilization. We have assembled a library of well characterized plant oligosaccharides produced either by partial hydrolysis from polysaccharides or by de novo chemical synthesis. Once coupled to protein, these neoglycoconjugates are versatile reagents that can be printed as microarrays onto a variety of slide types and membranes. We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes.

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