Cytidylate kinase (prokaryote)

Reference code: E-CMPK

500 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

500 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

Kinase

EC Number:

2.7.4.25

Synonyms:

(d)CMP kinase; ATP:CMP phosphotransferase; UMP-CMP kinase

Source:

Prokaryote

Molecular Weight:

26,910

Concentration:

Supplied at ~ 125 U/mL

Expression:

Recombinant from a Prokaryotic source

Specificity:

Catalyses the reaction:
ATP + (d)CMP = ADP + (d)CDP
The prokaryotic cytidine monophosphate kinase specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor.

Specific Activity:

~ 50 U/mg (25^oC, pH 7.6 on GMP)

Unit Definition:

One Unit of cytidylate kinase is defined as the amount of enzyme required to produce one µmole of CDP from CMP and ATP per minute, in the presence of NADH, in TEA buffer (73 mM), pH 7.6 at 25^oC.

Temperature Optima:

25^oC

pH Optima:

7.6

Application examples:

Applications in the measurement of CMP.

This product has been discontinued (read more).

High purity recombinant Cytidylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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