50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Prices exclude VAT
This product has been discontinued (read more)
|Content:||50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)|
|Storage Temperature:|| Short term stability: 2-8oC, |
Long term stability: See individual component labels
|Stability:||> 2 years under recommended storage conditions|
|Assay Format:||Spectrophotometer, Microplate, Auto-analyser|
|Linear Range:||10 to 150 µg of aspartame per assay|
|Limit of Detection:||0.57 mg/L|
|Reaction Time (min):||~ 5 min|
|Application examples:||Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials.|
|Method recognition:||Novel method|
This product has been discontinued (read more).
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
We have a wide range of other assay kit products.
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Measures aspartame and breakdown products (L-aspartate and aspartame acid)
- Very specific
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.
As commonly recognized, the excretion of acetate by the aerobic growth of Escherichia coli on glucose is a manifestation of imbalanced flux between glycolysis and the tricarboxylic acid (TCA) cycle. Accordingly, this may restrict the production of recombinant proteins in E. coli, due to the limited amounts of precursor metabolites produced in TCA cycle. To approach this issue, an extra supply of intermediate metabolites in TCA cycle was made by conversion of aspartate to fumarate, a reaction mediated by the activity of L-aspartate ammonia-lyase (aspartase). As a result, in the glucose minimal medium containing aspartate, the production of two recombinant proteins, β-galactosidase and green fluorescent protein, in the aspartase-producing strain was substantially increased by 5-fold in association with 30–40% more biomass production. This preliminary study illustrates the great promise of this approach used to enhance the production of these two recombinant proteins.Hide Abstract