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Aspartame Assay Kit

Product code: K-ASPTM

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Prices exclude VAT

This product has been discontinued

Content: 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Aspartame
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 10 to 150 µg of aspartame per assay
Limit of Detection: 0.57 mg/L
Reaction Time (min): ~ 5 min
Application examples: Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials.
Method recognition: Novel method

This product has been discontinued (read more).

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

We have a wide range of other assay kit products.

Scheme-K-ASPTM ASPTM megazyme

  • Very cost effective 
  • All reagents stable for > 12 months after preparation 
  • Only enzymatic kit available 
  • Measures aspartame and breakdown products (L-aspartate and aspartame acid) 
  • Very specific 
  • Very rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats
Certificate of Analysis
Safety Data Sheet
FAQs Assay Protocol Data Calculator Product Performance
Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase.

Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.

As commonly recognized, the excretion of acetate by the aerobic growth of Escherichia coli on glucose is a manifestation of imbalanced flux between glycolysis and the tricarboxylic acid (TCA) cycle. Accordingly, this may restrict the production of recombinant proteins in E. coli, due to the limited amounts of precursor metabolites produced in TCA cycle. To approach this issue, an extra supply of intermediate metabolites in TCA cycle was made by conversion of aspartate to fumarate, a reaction mediated by the activity of L-aspartate ammonia-lyase (aspartase). As a result, in the glucose minimal medium containing aspartate, the production of two recombinant proteins, β-galactosidase and green fluorescent protein, in the aspartase-producing strain was substantially increased by 5-fold in association with 30–40% more biomass production. This preliminary study illustrates the great promise of this approach used to enhance the production of these two recombinant proteins.

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Safety Information
Symbol : GHS07
Signal Word : Warning
Hazard Statements : H315, H319
Precautionary Statements : P264, P270, P280, P301+P312, P302+P352, P305+P351+P338, P321, P330, P332+P313, P501
Safety Data Sheet
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