Aspartame Assay Kit

Reference code: K-ASPTM

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

This product has been discontinued

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Certificate of Analysis

Product Performance

Content:

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,
Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Aspartame

Assay Format:

Spectrophotometer, Microplate, Auto-analyser

Detection Method:

Absorbance

Wavelength (nm):

340

Signal Response:

Decrease

Linear Range:

10 to 150 µg of aspartame per assay

Limit of Detection:

0.57 mg/L

Reaction Time (min):

~ 5 min

Application examples:

Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials.

Method recognition:

Novel method

Advantages

Very cost effective
All reagents stable for > 12 months after preparation
Only enzymatic kit available
Measures aspartame and breakdown products (L-aspartate and aspartame acid)
Very specific
Very rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats

This product has been discontinued (read more).

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuant^TM Wave Spectrophotometer (D-MQWAVE).

We have a wide range of other assay kit products.

Scheme-K-ASPTM ASPTM megazyme

Publications

Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase.

Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.

Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase.

Wang, Z. W., Chen, Y. & Chao, Y. P. (2006). Journal of Biotechnology, 124 (2), 403-411.

As commonly recognized, the excretion of acetate by the aerobic growth of Escherichia coli on glucose is a manifestation of imbalanced flux between glycolysis and the tricarboxylic acid (TCA) cycle. Accordingly, this may restrict the production of recombinant proteins in E. coli, due to the limited amounts of precursor metabolites produced in TCA cycle. To approach this issue, an extra supply of intermediate metabolites in TCA cycle was made by conversion of aspartate to fumarate, a reaction mediated by the activity of L-aspartate ammonia-lyase (aspartase). As a result, in the glucose minimal medium containing aspartate, the production of two recombinant proteins, β-galactosidase and green fluorescent protein, in the aspartase-producing strain was substantially increased by 5-fold in association with 30–40% more biomass production. This preliminary study illustrates the great promise of this approach used to enhance the production of these two recombinant proteins.

Link to Article