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Product code: E-GERF
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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||> 4 years at 4oC|
|Synonyms:||glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase|
|Concentration:||Supplied at ~ 370 U/mL|
|Expression:||Recombinant from Ruminococcus flavefaciens|
|Specificity:||Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.|
|Specific Activity:||~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3)|
|Unit Definition:||One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3.|
|Application examples:||Applications in carbohydrate research and in the food industry.|
High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.