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Fructanase Mixture
(Ultrapure, recombinant, liquid)

Fructanase Mixture Ultrapure recombinant liquid E-FRLQPU
Product code: E-FRLQPU
€260.00

10 mL / 20,000 Units

Prices exclude VAT

Available for shipping

Content: 10 mL / 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: In 50% (v/v) glycerol plus 0.02% sodium azide
Physical Form: Solution
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus niger
Concentration: endo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 10,000 U
Expression: Recombinant from Aspergillus niger
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 60oC
pH Optima: 4.5
Application examples: Applications in carbohydrate research and in the food industry.
Method recognition: AOAC Method 997.08 and AOAC Method 2000.11

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Componentsexo-Inulinase (2,000 U/mL on kestose, at 40oC) and endo-Inulinase (100 U/mL on inulin at 40oC).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Validation of Method

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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