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α-L-Arabinofuranosidase (Cellvibrio japonicus)

alpha-L-Arabinofuranosidase Cellvibrio japonicus E-ABFCJ
Product code: E-ABFCJ
€165.00

500 Units on pNP-α-Araf at 40oC

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Available for shipping

Content: 500 Units on pNP-α-Araat 40oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: α-Arabinofuranosidase
EC Number: 3.2.1.55
CAZy Family: GH51
CAS Number: 9067-74-7
Synonyms: non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
Source: Cellvibrio japonicus
Molecular Weight: 55,700
Concentration: Supplied at ~ 250 U/mL
Expression: Recombinant from Cellvibrio japonicus
Specificity: Hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses α-1,5-linked arabino-oligosaccharides at a much lower rate.
Specific Activity: ~ 12 U/mg (40oC, pH 5.5 on p-nitrophenyl-α-L-arabinofuranoside)
Unit Definition: One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (2.5 mM) in sodium acetate buffer (100 mM), pH 5.5 at 40oC.
Temperature Optima: 50oC
pH Optima: 5.5
Application examples: Applications in carbohydrate and biofuels research.

High purity recombinant α-L-Arabinofuranosidase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Arabinoxylanase from glycoside hydrolase family 5 is a selective enzyme for production of specific arabinoxylooligosaccharides.

Falck, P., Linares-Pastén, J. A., Karlsson, E. N. & Adlercreutz, P. (2017). Food Chemistry, In Press.

An arabinose specific xylanase from glycoside hydrolase family 5 (GH5) was used to hydrolyse wheat and rye arabinoxylan, and the product profile showed that it produced arabinose substituted oligosaccharides (AXOS) having 2-10 xylose residues in the main chain but no unsubstituted xylooligosaccharides (XOS). Molecular modelling showed that the active site has an open structure and that the hydroxyl groups of all xylose residues in the active site are solvent exposed, indicating that arabinose substituents can be accommodated in the glycone as well as the aglycone subsites. The arabinoxylan hydrolysates obtained with the GH5 enzyme stimulated growth of Bifidobacterium adolescentis but not of Lactobacillus brevis. This arabinoxylanase is thus a good tool for the production of selective prebiotics.

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Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
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