40 assays of each per kit
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|Content:||40 assays of each per kit|
Short term stability: 2-8oC,
Long term stability: See individual component labels
|Stability:||> 2 years under recommended storage conditions|
|Analyte:||Digestible Starch, Resistant Starch, Total Starch|
|Linear Range:||5 to 100 µg of D-glucose per assay|
|Limit of Detection:||2 g/100 g|
|Reaction Time (min):||~ 360 min|
|Application examples:||Plant materials, starch samples and other materials.|
The Digestible and Resistant Starch Assay Kit (K-DSTRS) method is based on the research of Englyst et al. (Ref) with some modifications. Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal.
In line with Englyst definitions:
Rapidly digestible starch (RDS) is that starch which is digested within 20 min.
Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.
A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).
Resistant starch (RS) then, is that starch which is not digested within 4 h.
The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009.