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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||Minimum 1 year at 4oC. Check vial for details.|
|Enzyme Activity:||Other Activities|
|Synonyms:||acetate-CoA ligase; acetate:CoA ligase (AMP-forming)|
|Concentration:||Supplied at ~ 125 U/mL|
|Expression:||Recombinant from Bacillus Subtilis|
Catalyses the reaction:
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
~ 7.6 U/mg (37oC, pH 7.5 on acetic acid);
~ 39 U/mg (37oC, pH 8.4 on acetic acid);
~ 7.0 U/mg (25oC, pH 7.5 on acetic acid)
|Unit Definition:||One Unit of acetyl-CoA synthetase activity is defined as the amount of enzyme required to produce one µmole of acetyl coenzyme A from acetic acid, ATP and coenzyme A per min in the presence of NAD+ in TEA buffer (128 mM), pH 8.4 at 25oC.|
|Application examples:||Applications in the analysis and measurement of acetic acid.|
High purity recombinant Acetyl-CoA synthetase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
See our complete list of analytical enzymes.
Certificate of Analysis
Safety Data Sheet
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