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4-Nitrophenyl-β-maltotrioside

4-Nitrophenyl-beta-maltotrioside O-PNPC3-100
Product code: O-PNPC3-100
€218.00

100 mg

Prices exclude VAT

Available for shipping

Content: 100 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Solid
Stability: > 10 years under recommended storage conditions
CAS Number: 66451-58-9
Molecular Formula: C24H35NO18
Molecular Weight: 625.5
Purity: > 95%
Substrate For (Enzyme): β-Amylase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-β-maltotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A substrate for the measurement of β-amylase.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research.

Pedersen, H. L., Fangel, J. U., McCleary, B., Ruzanski, C., Rydahl, M. G., Ralet, M. C., Farkas, V., Von Schantz, L., Marcus, S. E., Andersen, M.C. F., Field, R., Ohlin, M., Knox, J. P., Clausen, M. H. & Willats, W. G. T. (2012). Journal of Biological Chemistry, 287(47), 39429-39438.

Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts of analytes. Nucleotide microarrays are well established in plant research, but carbohydrate microarrays are much less established, and one reason for this is a lack of suitable glycans with which to populate arrays. Polysaccharide microarrays are relatively easy to produce because of the ease of immobilizing large polymers noncovalently onto a variety of microarray surfaces, but they lack analytical resolution because polysaccharides often contain multiple distinct carbohydrate substructures. Microarrays of defined oligosaccharides potentially overcome this problem but are harder to produce because oligosaccharides usually require coupling prior to immobilization. We have assembled a library of well characterized plant oligosaccharides produced either by partial hydrolysis from polysaccharides or by de novo chemical synthesis. Once coupled to protein, these neoglycoconjugates are versatile reagents that can be printed as microarrays onto a variety of slide types and membranes. We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes.

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Safety Information
Symbol : Not Applicable
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Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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